TY  - JOUR
TI  - Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
AU  - Tonoyan, Lilit 
AU  -  Guihéneuf, Freddy 
AU  -  Friel, Ruari 
AU  - O'Flaherty, Vincent 
VL  - 10
IS  - 21
PY  - 2020
DA  - 2020/11/05
SP  - e3813
C1  - Bio-protocol 2020;10:e3813
DO  - 10.21769/BioProtoc.3813
UR  - https://doi.org/10.21769/BioProtoc.3813
AB  - This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person. It provides a platform for parallel, long-term studies of evolution and adaptation of microorganisms under the stress of antimicrobial agents and/or toxic pollutants. The system complies with the varied needs of researchers for an accessible, highly-throughput and reliable tool that is nevertheless easy to construct, use and operate, and not demanding of space, materials, medium supply and workload. Here, we also validate the use of this system to generate de novo resistance towards a novel antimicrobial and a commonly used antibiotic in an antimicrobial-sensitive model organism. We believe that this "Do It Yourself" (DIY) system may constitute a useful tool to address the global problem of antibiotic resistance and to develop non-antibiotic based therapies.
KW  - Continuous culturing
KW  - Chemostat
KW  - Multiplex platform
KW  - Adaptive laboratory evolution
KW  - Antimicrobial resistance
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False