TY  - JOUR
TI  - Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
AU  - Aguillera , Anabella 
AU  - Steelheart, Charlotte 
AU  - Alegre, Matías 
AU  - Berdun, Federico  
AU  - Salerno, Graciela 
AU  - Bartoli, Carlos  
AU  - Pagnussat, Gabriela  Carolina 
AU  - Martin, María  Victoria 
VL  - 10
IS  - 20
PY  - 2020
DA  - 2020/10/20
SP  - e3800
C1  - Bio-protocol 2020;10:e3800
DO  - 10.21769/BioProtoc.3800
UR  - https://doi.org/10.21769/BioProtoc.3800
AB  - Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.
KW  - Cyanobacteria
KW  - Antioxidants
KW  - Glutathione
KW  - Ascorbic acid
KW  - Oxidative stress
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False