TY  - JOUR
TI  - Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
AU  - Suzuki, Kohei 
AU  - Okamoto, Ryuichi 
VL  - 10
IS  - 19
PY  - 2020
DA  - 2020/10/05
SP  - e3775
C1  - Bio-protocol 2020;10:e3775
DO  - 10.21769/BioProtoc.3775
UR  - https://doi.org/10.21769/BioProtoc.3775
AB  - In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for single-cell analysis as a further application. This protocol should provide investigators sufficient tools to generate human organoids from biopsy samples and to accomplish a stable in-vitro assay system.
KW  - Organoid
KW  - Intestinal stem cell
KW  - Single-cell analysis
KW  - Endoscopic biopsy
KW  - Multiplex PCR
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False