TY  - JOUR
TI  - Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation
AU  - Hallinan, Grace I
AU  - Lopez, Dianne M
AU  - Vargas-Caballero, Mariana 
AU  - West, Jonathan 
AU  - Deinhardt, Katrin 
VL  - 10
IS  - 16
PY  - 2020
DA  - 2020/08/20
SP  - e3718
C1  - Bio-protocol 2020;10:e3718
DO  - 10.21769/BioProtoc.3718
UR  - https://doi.org/10.21769/BioProtoc.3718
AB  - The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of tau pathology propagation across circuits is a focus of intense research and has been investigated in vivo in human post-mortem brain and in mouse models of the diseases, in vitro in diverse cellular systems including primary neurons, and in cell free assays using purified recombinant tau protein. Here we describe a protocol that takes advantage of a minimalistic neuronal circuit arrayed within a microfluidic device to follow the propagation of tau misfolding from a presynaptic to a postsynaptic neuron. This assay allows high-resolution imaging as well as individual manipulation of the releasing and receiving neuron, and is therefore beneficial for investigating the propagation of tau and other misfolded proteins in vitro.
KW  - Protein misfolding propagation
KW  - Microfluidic device
KW  - Hippocampal culture
KW  - Tau
KW  - Imaging
KW  - Image analysis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False