TY  - JOUR
TI  - Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells
AU  - Kumar, Akhilesh 
AU  - D’Souza, Saritha S.
AU  - Uenishi, Gene 
AU  - Park, Mi Ae
AU  - Lee, Jeong Hee
AU  - Slukvin, Igor I.
VL  - 10
IS  - 13
PY  - 2020
DA  - 2020/07/05
SP  - e3675
C1  - Bio-protocol 2020;10:e3675
DO  - 10.21769/BioProtoc.3675
UR  - https://doi.org/10.21769/BioProtoc.3675
AB  - Pluripotent stem cells (PSCs) have the potential to provide homogeneous cell populations of T cells that can be grown at a clinical scale and genetically engineered to meet specific clinical needs. OP9-DLL4, a stromal line ectopically expressing the Notch ligand Delta-like 4 (DLL4) is used to support differentiation of PSCs to T-lymphocytes. This article outlines several protocols related to generation of T cells from human and non-human primate (NHP) PSCs, including initial hematopoietic differentiation of PSC on OP9 feeders or defined conditions, followed by coculture of the OP9-DLL4 cells with the PSC-derived hematopoietic progenitors (HPs), leading to efficient differentiation to T lymphocytes. In addition, we describe a protocol for robust T cell generation from hPSCs conditionally expressing ETS1. The presented protocols provide a platform for T cell production for disease modeling and evaluating their use for immunotherapy in large animal models.
KW  - Human pluripotent stem cells
KW  - Human embryonic stem cells
KW  - Non-human primate pluripotent stem cells
KW  - Hemogenic endothelium
KW  - Hematopoietic progenitor
KW  - T cells
KW  - Hematopoietic differentiation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False