TY  - JOUR
TI  - Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
AU  - Zhang, Chaozheng 
AU  - Liu, An 
AU  - Zhang, Tianshuang 
AU  - Li, Yi 
AU  - Zhao, Hua 
VL  - 10
IS  - 13
PY  - 2020
DA  - 2020/07/05
SP  - e3672
C1  - Bio-protocol 2020;10:e3672
DO  - 10.21769/BioProtoc.3672
UR  - https://doi.org/10.21769/BioProtoc.3672
AB  - Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and more researchers concentrate on SCFAs. This protocol describes, a direct injection gas chromatography detection method with a pretreatment method for extracting SCFA from mice feces by combining acidification. The corresponding sample limit of quantization (LOQ) and limit of detection (LOD) are 0.8-1.0 mg/L and 0.5-0.8 mg/L, respectively. The correlation coefficient of calibration curve is greater than 0.999. The recovery rate of the spiked standard is 80%-102%. This method can be used to analyze and determine SCFAs in mice feces. Therefore, this is an economical, effective and reproducible method for SCFAs measurement in mice samples.
KW  - Short-chain fatty acids
KW  - Acidification
KW  - Extraction
KW  - Gas chromatography
KW  - Biological samples
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False