TY  - JOUR
TI  - Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
AU  - Zhang, Zhenguang 
AU  - Loudon, Andrew S. I.
VL  - 10
IS  - 8
PY  - 2020
DA  - 2020/04/20
SP  - e3590
C1  - Bio-protocol 2020;10:e3590
DO  - 10.21769/BioProtoc.3590
UR  - https://doi.org/10.21769/BioProtoc.3590
AB  - Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen tissues and is highly reproduciable.
KW  - Club cell
KW  - Lung
KW  - Circadian clock
KW  - RNA seq
KW  - Laser capture micro-dissection
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False