TY  - JOUR
TI  - Isolation and Stimulation of Peritoneal Macrophages with  Apoptotic Jurkat Cells to Produce IL-10
AU  - Song, Mei 
AU  - Ma, Xiaojing  
VL  - 9
IS  - 24
PY  - 2019
DA  - 2019/12/20
SP  - e3467
C1  - Bio-protocol 2019;9:e3467
DO  - 10.21769/BioProtoc.3467
UR  - https://doi.org/10.21769/BioProtoc.3467
AB  - Clearance of apoptotic cells by macrophages is critical to ensuring cellular homeostasis and suppression of autoimmunity. Macrophage recognition of apoptotic cells triggers an anti-inflammatory response, which is mediated by the release of IL-10, TGF-β etc. with concurrent inhibition of pro-inflammatory cytokines (such as TNFα, IL-12, IL-1β). To characterize cytokine profile produced by macrophages during phagocytosis of apoptotic cells, we developed an effective, more physiologic system using isolated murine peritoneal macrophages and T-lymphocyte cell line Jurkat as a source of apoptotic cells. Apoptosis of Jurkat cells is induced with staurosporine, a protein kinase C (PKC) inhibitor and detected by Annexin V/propidium iodide staining. This in vitro assay demonstrates that murine peritoneal macrophages produce large amounts of IL-10 following exposure to apoptotic Jurkat cells.
KW  - Apoptosis
KW  - Macrophage
KW  - Jurkat
KW  - IL-10
KW  - Phagocytosis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False