TY  - JOUR
TI  - High Resolution Respirometry in Candida albicans
AU  - Duvenage, Lucian 
AU  - Munro, Carol A
AU  - Gourlay, Campbell  W
VL  - 9
IS  - 17
PY  - 2019
DA  - 2019/09/05
SP  - e3361
C1  - Bio-protocol 2019;9:e3361
DO  - 10.21769/BioProtoc.3361
UR  - https://doi.org/10.21769/BioProtoc.3361
AB  - Many Candida species, such as the opportunistic human pathogen Candida albicans, are Crabtree-Negative yeasts and are therefore highly dependent on the energy generated through oxidative phosphorylation. Respiration control is linked to a range of aspects of C. albicans cell physiology that appear to be important for virulence, most notably its ability to switch from yeast to hyphal forms and the maintenance of the cell wall. The following protocol allows for the measurement and characterization of respiration in C. albicans using high resolution respirometry. We outline how addition of respiration inhibitors can be used to assay the “mode” of respiration, mitochondrial health and the level of electron transport that is coupled to ATP synthase activity in living cell cultures. These data provide useful insight into the effects of external factors, such as exposure to anti-fungal compounds, or internal changes such as genetic alterations on respiratory performance.
KW  - Candida
KW  - Respiration
KW  - Mitochondria
KW  - Respirometry
KW  - Yeast
KW  - Metabolism
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False