TY  - JOUR
TI  - In vitro Culture of Human PBMCs
AU  - Panda, Santosh K
AU  - Ravindran, Balachandran 
VL  - 3
IS  - 3
PY  - 2013
DA  - 2013/02/05
SP  - e322
C1  - Bio-protocol 2013;3:e322
DO  - 10.21769/BioProtoc.322
UR  - https://doi.org/10.21769/BioProtoc.322
AB  - Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitisation in antigen recall assays by scoring lymphocyte proliferation, c) immunophenotyping for surface markers as well as intracellular molecules in monocytes and lymphocytes etc. Activation of monocytes/macrophages by small molecules, cytokines and pathogen components can also be monitored. PBMCs can also be used for a variety of structural and functional studies for addressing issues in human immunology such as scoring for apoptosis and production of cytokines as well as other mediators in vitro.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False