TY  - JOUR
TI  - Early Life Ovalbumin Sensitization and Aerosol Challenge for the Induction of Allergic Airway Inflammation in a BALB/c Murine Model
AU  - Mincham, Kyle T
AU  - Scott, Naomi M
AU  - Lauzon-Joset, Jean-Francois 
AU  - Leffler, Jonatan 
AU  - Stumbles, Philip A
AU  - Holt, Patrick G
AU  - Strickland, Deborah H
VL  - 9
IS  - 5
PY  - 2019
DA  - 2019/03/05
SP  - e3181
C1  - Bio-protocol 2019;9:e3181
DO  - 10.21769/BioProtoc.3181
UR  - https://doi.org/10.21769/BioProtoc.3181
AB  - The early life period represents a time of immunological plasticity whereby the functionally immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of allergic asthmatic disease. Murine models are widely used as an investigative tool to examine the pathophysiology of allergic asthma; however, models in current usage typically do not encapsulate the early life period which represents the time of maximal risk for disease inception in humans. To address this issue, this protocol adapted an experimental animal model of disease for sensitization to ovalbumin during the immediate post-weaning period beginning at 21 days of age. By initially sensitizing mice during this early life post-weaning period, researchers can more closely align experimental allergic airway disease models with the human age group most at risk for asthma development.
KW  - Asthma
KW  - Allergy
KW  - Airway inflammation
KW  - Ovalbumin
KW  - Sensitization
KW  - Early life
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False