TY  - JOUR
TI  - Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
AU  - Bao, Yan 
AU  - Bassham, Diane C.
VL  - 8
IS  - 23
PY  - 2018
DA  - 2018/12/05
SP  - e3101
C1  - Bio-protocol 2018;8:e3101
DO  - 10.21769/BioProtoc.3101
UR  - https://doi.org/10.21769/BioProtoc.3101
AB  - Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol (DTT)-mediated root growth inhibition, analysis of splicing of the UPR-responsive transcription factor bZIP60 (basic Leucine Zipper Domain 60), and upregulation of relevant UPR genes. We provide here a quick and robust method to detect UPR signaling in Arabidopsis thaliana protoplasts. This assay can also be applied to other plant species for which protoplasts can be isolated.
KW  - Arabidopsis
KW  - bZIP60 splicing
KW  - DTT
KW  - ER stress
KW  - Protoplasts
KW  - Tm
KW  - UPR
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False