TY  - JOUR
TI  - Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer
AU  - Dasgupta, Agnik 
AU  - Jacob, Andrew E.
AU  - Amack, Jeffrey D.
VL  - 8
IS  - 22
PY  - 2018
DA  - 2018/11/20
SP  - e3090
C1  - Bio-protocol 2018;8:e3090
DO  - 10.21769/BioProtoc.3090
UR  - https://doi.org/10.21769/BioProtoc.3090
AB  - A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors during LRO formation. To address this, we developed a Cre-loxP based method to mosaically label precursor cells, called dorsal forerunner cells, that give rise to the zebrafish LRO known as Kupffer’s vesicle. This methodology allows lineage tracing, 3-dimensional (3D) reconstruction and morphometric analysis of single LRO cells in living embryos. The ability to visualize and quantify individual LRO cell dynamics provides an opportunity to advance our understanding of LRO development, and in a broader sense, investigate the interplay between intrinsic biochemical mechanisms and extrinsic mechanical forces that drive morphogenesis of epithelial tissues.
KW  - Left-right organizer
KW  - Dorsal forerunner cells
KW  - Kupffer’s vesicle
KW  - Mosaic labeling
KW  - Zebrafish embryo
KW  - 3D reconstructions
KW  - Single cell
KW  - Morphometric analysis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False