TY  - JOUR
TI  - Ex vivo Culture and Lentiviral Transduction of Benign Prostatic Hyperplasia (BPH) Samples
AU  - McClurg, Urszula Lucja
AU  - McCracken, Stuart R
AU  - Butler, Lisa 
AU  - Riabowol, Karl T
AU  - Binda, Olivier 
VL  - 8
IS  - 21
PY  - 2018
DA  - 2018/11/05
SP  - e3075
C1  - Bio-protocol 2018;8:e3075
DO  - 10.21769/BioProtoc.3075
UR  - https://doi.org/10.21769/BioProtoc.3075
AB  - To assess oncogenic potential, classical transformation assays are based on cell line models. However, cell line based models do not reflect the complexity of human tissues. We thus developed an inducible expression system for gene expression in ex vivo human tissues, which maintain native tissue architecture, such as epithelia and stroma. To validate the system, we transduced and expressed known tumor suppressors (p53, p33ING1b), oncoproteins (RasV12, p47ING3), or controls (empty vector, YFP) in ex vivo prostate tissues, then assessed proliferation by immunohistochemistry of markers (H3S10phos). Herein, we describe how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.
KW  - Ex vivo tissue culture model
KW  - Proliferation
KW  - Oncogenic
KW  - Transformation
KW  - Prostate
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False