TY  - JOUR
TI  - In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals
AU  - Le Guerneve, Christine 
AU  - Becquer, Adeline  
AU  - Torres-Aquino, Margarita  
AU  - Amenc, Laurie K
AU  - Trives-Segura, Carlos 
AU  - Staunton, Siobhan  
AU  - Plassard, Claude 
AU  - Quiquampoix, Hervé  
VL  - 8
IS  - 16
PY  - 2018
DA  - 2018/08/20
SP  - e2973
C1  - Bio-protocol 2018;8:e2973
DO  - 10.21769/BioProtoc.2973
UR  - https://doi.org/10.21769/BioProtoc.2973
AB  - We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host (Pinus pinaster) or non-host (Zea mays) plants. A device was developed for the in vivo studies allowing the circulation of a continuously oxygenated mineral solution in an NMR tube containing the mycelia. The in vitro studies were performed on fungal material after several consecutive treatment steps (freezing in liquid nitrogen; crushing with perchloric acid; elimination of perchloric acid; freeze-drying; dissolution in an appropriate liquid medium).
KW  - 31P-NMR spectroscopy
KW  - Phosphate compartmentation
KW  - Polyphosphate metabolism
KW  - Ectomycorrhizal fungi
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False