TY  - JOUR
TI  - Hypoxia Reporter Element Assay
AU  - Buelow, Daelynn R
AU  - Baker, Sharyn D. D
VL  - 8
IS  - 15
PY  - 2018
DA  - 2018/08/05
SP  - e2951
C1  - Bio-protocol 2018;8:e2951
DO  - 10.21769/BioProtoc.2951
UR  - https://doi.org/10.21769/BioProtoc.2951
AB  - Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the simultaneous measurement of two different genes within a single cell, thus improving experimental accuracy. Within this protocol, we describe the utilization of the LightSwitch Dual Assay System to measure BMX expression in response to hypoxic conditions.
KW  - Luciferase
KW  - Reporter assay
KW  - Hypoxia
KW  - Regulation
KW  - Transcription
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False