TY  - JOUR
TI  - HCV Reporter System (Viral Infection-Activated Split-Intein-Mediated  Reporter System) for Testing Virus Cell-to-cell Transmission ex-vivo
AU  - Zhao, Fanfan 
AU  - Zhao, Ting 
AU  - Deng, Libin 
AU  - Lv, Dawei 
AU  - Zhang, Xiaolong 
AU  - Pan, Xiaoyu 
AU  - Xu, Jun 
AU  - Long, Gang 
VL  - 8
IS  - 15
PY  - 2018
DA  - 2018/08/05
SP  - e2949
C1  - Bio-protocol 2018;8:e2949
DO  - 10.21769/BioProtoc.2949
UR  - https://doi.org/10.21769/BioProtoc.2949
AB  - Hepatitis C virus (HCV) spread involves two distinct entry pathways: cell-free transmission and cell-to-cell transmission. Cell-to-cell transmission is not only an efficient way for viruses to spread but also an effective method for escaping neutralizing antibodies. We adapted the viral infection-activated split-intein-mediated reporter system (VISI) and developed a straightforward model for Live-cell monitoring of HCV cell-to-cell transmission ex-vivo: co-culture of HCV infected donor cells (red signal) with uninfected recipient cells (green signal) and elimination of the cell-free transmission by adding potent neutralizing antibody AR3A in the supernatant. With this model, the efficiency of cell-to-cell transmission can be evaluated by counting the number of foci designated by the green signal of recipient cells.
KW  - HCV
KW  - Reporter system
KW  - Fluorescence signal
KW  - Virus spread
KW  - Cell-to-cell transmission
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False