TY  - JOUR
TI  - Brain Tissue Culture of Per2::Luciferase Transgenic Mice for ex vivo Bioluminescence
AU  - Salaberry, Nora L.
AU  - Mendoza, Jorge 
VL  - 8
IS  - 13
PY  - 2018
DA  - 2018/07/05
SP  - e2917
C1  - Bio-protocol 2018;8:e2917
DO  - 10.21769/BioProtoc.2917
UR  - https://doi.org/10.21769/BioProtoc.2917
AB  - In circadian research, it is essential to be able to track a biological rhythm for several days with the minimum perturbation for the organisms or tissues. The use of transgenic mice lines, in which the luciferase reporter is coupled to a molecular clock protein (here PERIOD2), gives us the opportunity to follow the circadian activity in different tissues or even single clock cells for days without manipulation. This method creates sections using a mouse brain matrix, which allows us to obtain several brain samples quickly at a single time point.
KW  - Bioluminescence
KW  - Per2::Luciferase
KW  - Brain
KW  - Mouse matrix
KW  - Tissue culture
KW  - Rhythm
KW  - Circadian
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False