TY  - JOUR
TI  - Enzymatic Activity Assay for Invertase in Synechocystis Cells
AU  - Tan, Xiaoming 
AU  - Song, Kuo 
AU  - Lu, Xuefeng 
VL  - 8
IS  - 10
PY  - 2018
DA  - 2018/05/20
SP  - e2856
C1  - Bio-protocol 2018;8:e2856
DO  - 10.21769/BioProtoc.2856
UR  - https://doi.org/10.21769/BioProtoc.2856
AB  - Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be determined. All the methods for determination of the invertase activity are dependent on detection of the glucose product generated by the invertase. Here we describe an ion chromatography based protocol of our laboratory for determination of cyanobacterial intracellular invertase activity.
KW  - Cyanobacteria
KW  - Synechocystis
KW  - Invertase enzymatic activity
KW  - Sucrose
KW  - Ion chromatography
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False