TY  - JOUR
TI  - Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier
AU  - Call, Mindy  
AU  - Meyer, Ewa Anna
AU  - Kao, Winston W.
AU  - Kruse, Friedrich E
AU  - Schloetzer-Schredhardt, Ursula 
VL  - 8
IS  - 10
PY  - 2018
DA  - 2018/05/20
SP  - e2849
C1  - Bio-protocol 2018;8:e2849
DO  - 10.21769/BioProtoc.2849
UR  - https://doi.org/10.21769/BioProtoc.2849
AB  - The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice expressing a dual reporter gene under the control of the Tet-on system and the keratin 12 promoter (Meyer-Blazejewska et al., 2011). A clonal growth assay was performed to enrich for stem cells. Once holoclones formed they were transferred onto a fibrin carrier and cultivated to obtain a confluent epithelial cell layer. Limbal stem cell deficient (LSCD) mice were used as the transplant recipient in order to test for successful grafting and eventual differentiation into a corneal epithelial phenotype.
KW  - Holoclones
KW  - Clonal growth
KW  - Hair follicle stem cells
KW  - Transgenic mice
KW  - Fibrin carrier
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False