TY  - JOUR
TI  - Measurement of Intracellular ROS in Caenorhabditis elegans Using  2’,7’-Dichlorodihydrofluorescein Diacetate
AU  - Yoon, Dong Suk
AU  - Lee, Myon-Hee 
AU  - Cha, Dong Seok
VL  - 8
IS  - 6
PY  - 2018
DA  - 2018/03/20
SP  - e2774
C1  - Bio-protocol 2018;8:e2774
DO  - 10.21769/BioProtoc.2774
UR  - https://doi.org/10.21769/BioProtoc.2774
AB  - Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, cardiovascular disease, ischemia-reperfusion and aging. Over the past several decades, antioxidants have received explosive attention regarding their protective potential against these deleterious reactions. Accordingly, many analytical methodologies have been developed for the evaluation of the antioxidant capacity of compounds or complex biological samples. Herein, we introduce a simple and convenient method to detect in vivo intracellular ROS levels photometrically in Caenorhabditis elegans using 2’,7’-dichlorofluorescein diacetate (H2DCFDA), a cell permeant tracer.
KW  - Reactive oxygen species
KW  - 2’,7’-Dichlorofluorescein diacetate
KW  - C. elegans
KW  - Antioxidant
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False