TY  - JOUR
TI  - Measurement of Lysosomal Size and Lysosomal Marker  Intensities in Adult Caenorhabditis elegans
AU  - Huynh, Julie  M
AU  - Dang, Hope 
AU  - Fares, Hanna 
VL  - 8
IS  - 3
PY  - 2018
DA  - 2018/02/05
SP  - e2724
C1  - Bio-protocol 2018;8:e2724
DO  - 10.21769/BioProtoc.2724
UR  - https://doi.org/10.21769/BioProtoc.2724
AB  - Assays have been developed to study trafficking in various tissues of Caenorhabditis elegans. Adult C. elegans intestinal cells are large and have extensive endocytic networks, thus making them a good system for deciphering the endocytic pathway using live imaging techniques. However, the presence of auto-fluorescent gut granules in adult intestine can interfere with the signals of endocytic compartment reporters, like GFP. Here we demonstrate a protocol adapted from the original method developed by the Grant laboratory to identify signals from reporters in adult intestinal cells. The goal of this protocol is to identify endocytic compartments tagged with fluorescent markers without any confounding effects of background autofluorescent gut granules in adult intestinal cells of Caenorhabditis elegans.
KW  - C. elegans
KW  - Intestine
KW  - Endocytosis
KW  - Gut granule
KW  - Confocal microscopy
KW  - Lysosome
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False