TY  - JOUR
TI  - Culture and Nucleofection of Postnatal Day 7 Cortical and Cerebellar Mouse Astroglial Cells
AU  - Chouchane, Malek 
AU  - Costa, Marcos Romualdo
VL  - 8
IS  - 3
PY  - 2018
DA  - 2018/02/05
SP  - e2712
C1  - Bio-protocol 2018;8:e2712
DO  - 10.21769/BioProtoc.2712
UR  - https://doi.org/10.21769/BioProtoc.2712
AB  - Lineage reprogramming of astroglial cells isolated from different brain regions leads to the generation of different neuronal subtypes. This protocol describes the isolation and culture of neocortical and cerebellar astrocytes from postnatal mice. We also present a comprehensive description of the main steps towards successful gene delivery in these cells using nucleofection. Neocortex and cerebellum astrocyte cultures obtained with these methods are suitable for the study of molecular and cellular mechanisms involved in direct cell lineage reprogramming into induced neurons (iNs).
KW  - Primary cell culture
KW  - Murine astrocytes
KW  - Nucleofection
KW  - Cell lineage reprogramming
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False