TY  - JOUR
TI  - Determination of DNA Damage in the Retina Photoreceptors of Drosophila
AU  - Damulewicz, Milena  
AU  - Pyza, Elzbieta 
VL  - 8
IS  - 3
PY  - 2018
DA  - 2018/02/05
SP  - e2708
C1  - Bio-protocol 2018;8:e2708
DO  - 10.21769/BioProtoc.2708
UR  - https://doi.org/10.21769/BioProtoc.2708
AB  - The retina is sensitive for light damages, because of direct light exposure, especially intense blue and UV light, which increase level of ROS and other toxic phototransduction products in photoreceptor cells. In our previous work (Damulewicz et al., 2017a and 2017b), we used 8-oxo-deoxyguanosine (8-OHdG) as a marker for oxidative stress to investigate the role of heme oxygenase in DNA protection against UV light. In this protocol, we showed how to determine the level of DNA damages in the retina using immunohistochemical staining.
KW  - Oxidative stress
KW  - 8-Oxo-deoxyguanosine
KW  - UV light
KW  - Retina degeneration
KW  - DNA damage
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False