TY  - JOUR
TI  - Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
AU  - Tavakol, Shima 
AU  - Azedi, Freshteh 
AU  - Hoveizi, Elham 
AU  - Ai, Jafar 
AU  - Joghataei, Mohammad Taghi 
VL  - 8
IS  - 2
PY  - 2018
DA  - 2018/01/20
SP  - e2693
C1  - Bio-protocol 2018;8:e2693
DO  - 10.21769/BioProtoc.2693
UR  - https://doi.org/10.21769/BioProtoc.2693
AB  - Human endometrial stem cell/stromal cells (hEnSCs) are isolated from endometrium or menstrual blood and are recognized as a valuable cell type in tissue engineering and cell therapy. Furthermore, hEnSCs, which have CD90 (a mesenchymal marker), CD105 (endoglin), CD44, CD146 (endometrial stem cell markers) and lack CD31 (Endothelial marker), CD34 (hematopoietic marker) and CD133 on the cell surface, are a new source of mesenchymal stem/stromal cells. Additionally, these cells can be encapsulated into self-assembling peptide nanofibers as a 3D scaffold for applications in the treatment of neurodegenerative diseases. Here, we describe a protocol to isolate hEnSCs from endometrium or menstrual blood.
KW  - Endometrial stem cell
KW  - Endometrial stromal cell
KW  - Menstrual blood
KW  - Cell isolation
KW  - Neural differentiation
KW  - Self-assembling peptide nanofiber
KW  - 3D cell culture
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False