TY  - JOUR
TI  - Isolation and Purification of Viruses Infecting Cyanobacteria  Using a Liquid Bioassay Approach
AU  - Chénard , Caroline 
AU  - Chan, Amy M
VL  - 8
IS  - 2
PY  - 2018
DA  - 2018/01/20
SP  - e2691
C1  - Bio-protocol 2018;8:e2691
DO  - 10.21769/BioProtoc.2691
UR  - https://doi.org/10.21769/BioProtoc.2691
AB  - The following protocol describes the isolation and purification of viruses infecting cyanobacteria using a liquid bioassay approach. Viruses infecting cyanobacteria are also known as cyanophages. This protocol was written specifically for the isolation of cyanophages infecting freshwater cyanobacteria particularly, cyanobacteria that cannot be cultured on solid media. The use of a clonal cyanobacterial culture is recommended for the isolation of viruses. Growth conditions (i.e., media, light cycle and temperature) should be modified based on the host of interest.
KW  - Virus isolation
KW  - Virus purification
KW  - Aquatic microbiology
KW  - Cyanophage
KW  - Cyanobacteria
KW  - MPN assay
KW  - Liquid bioassay
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False