TY  - JOUR
TI  - Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
AU  - Jiang, Dongsheng 
AU  - de Vries, Juliane C.
AU  - Muschhammer, Jana 
AU  - Sindrilaru, Anca 
AU  - Scharffetter-Kochanek, Karin 
VL  - 7
IS  - 24
PY  - 2017
DA  - 2017/12/20
SP  - e2660
C1  - Bio-protocol 2017;7:e2660
DO  - 10.21769/BioProtoc.2660
UR  - https://doi.org/10.21769/BioProtoc.2660
AB  - Neutrophils are the most abundant leukocytes in the blood. In the recent decades, their crucial roles in host defense, immune regulation and tissue damage have been studied in a deeper dimension. In this protocol, we described a mouse model of immune complex-mediated vasculitis in the dorsal skin induced by Arthus reaction, and the subsequent analysis of edema, hemorrhage and tissue damage due to neutrophil activation by means of Evans blue area analysis, histology, and immunofluorescence. This protocol could facilitate the investigation of cellular therapy strategy against over-activated neutrophil-mediated tissue damage.
KW  - Vasculitis
KW  - Neutrophil
KW  - Evans blue
KW  - Tissue damage
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False