TY  - JOUR
TI  - In vivo Leukemogenesis Model Using Retrovirus Transduction
AU  - Okuda, Hiroshi 
AU  - Yokoyama, Akihiko 
VL  - 7
IS  - 23
PY  - 2017
DA  - 2017/12/05
SP  - e2627
C1  - Bio-protocol 2017;7:e2627
DO  - 10.21769/BioProtoc.2627
UR  - https://doi.org/10.21769/BioProtoc.2627
AB  - Various genetic alterations such as chromosomal translocation cause leukemia. For examples, gene rearrangements of the mixed-lineage leukemia (MLL) gene generate MLL fusion genes, whose products are potent oncogenic drivers in acute leukemia. To better understand the mechanism of disease onset, several murine leukemia models using retroviral gene transduction, xenograft, or Cre-mediated chromosomal translocation have been developed over the past twenty years. Particularly, a retroviral gene transduction-mediated murine leukemia model has been frequently used in the leukemia research field. Here, we describe the detailed protocol for this model.
KW  - Mixed lineage leukemia
KW  - Leukemogenesis
KW  - MLL fusion
KW  - Leukemia
KW  - Mouse model
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False