TY  - JOUR
TI  - A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions
AU  - Liu, Andrew  W
AU  - Aoki, Sho  
AU  - Wickens, Jeffery R
VL  - 7
IS  - 22
PY  - 2017
DA  - 2017/11/20
SP  - e2610
C1  - Bio-protocol 2017;7:e2610
DO  - 10.21769/BioProtoc.2610
UR  - https://doi.org/10.21769/BioProtoc.2610
AB  - Gelatin embedding of whole brains for sectioning is a critical procedure used in neuroscience to ensure all morphological and spatial details are preserved intact. Here, we describe an inexpensive, reproducible and efficient means to embed post-fixed brains ready for sectioning in gelatin within a week’s time. The sections obtained are distortion-free and their fragile internal structures preserved which can be used for serial reconstructions for lesion studies and mapping of viral expression after stereotaxic injections. In addition, the separation of adjacent slices into a series of 3-4 vials facilitates subsequent organization and assembly of serial sections at the mounting step.
KW  - Gelatin embedding
KW  - Serial reconstruction
KW  - Slide mounting
KW  - Lesion
KW  - Viral expression
KW  - Neuronal tracing
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False