TY  - JOUR
TI  - Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
AU  - Wen, Jinkun  
AU  - Tan, Dandan 
AU  - Li, Lixia  
AU  - Guo, Jiasong 
VL  - 7
IS  - 20
PY  - 2017
DA  - 2017/10/20
SP  - e2588
C1  - Bio-protocol 2017;7:e2588
DO  - 10.21769/BioProtoc.2588
UR  - https://doi.org/10.21769/BioProtoc.2588
AB  - Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of neonatal rats and purified by the treatment of cytosine arabinoside (AraC).
KW  - Schwann cell
KW  - Spinal nerve
KW  - Isolation
KW  - Purification
KW  - Rat
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False