TY  - JOUR
TI  - In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
AU  - Shafiee, Abbas  
AU  - Khosrotehrani, Kiarash 
VL  - 7
IS  - 20
PY  - 2017
DA  - 2017/10/20
SP  - e2587
C1  - Bio-protocol 2017;7:e2587
DO  - 10.21769/BioProtoc.2587
UR  - https://doi.org/10.21769/BioProtoc.2587
AB  - The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. Previous studies showed mesenchymal stem/stromal cells (MSCs) could improve the survival and capillary formation capacity of ECFCs in co-culture systems. In this article, we describe a protocol for in vitro co-culture of MSCs and ECFCs to prime ECFCs for better engraftment.
KW  - Endothelial colony forming cells
KW  - Endothelial progenitor cells
KW  - Mesenchymal stem/stromal cells
KW  - Vascularization
KW  - Placenta
KW  - Co-culture
KW  - in vitro
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False