TY  - JOUR
TI  - Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi  and Plants to Follow Fungal Phosphate Metabolism
AU  - Becquer, Adeline  
AU  - Torres-Aquino, Margarita  
AU  - Le Guernevé, Christine 
AU  - Amenc, Laurie K
AU  - Trives-Segura, Carlos 
AU  - Staunton, Siobhan  
AU  - Quiquampoix, Hervé  
AU  - Plassard, Claude 
VL  - 7
IS  - 20
PY  - 2017
DA  - 2017/10/20
SP  - e2577
C1  - Bio-protocol 2017;7:e2577
DO  - 10.21769/BioProtoc.2577
UR  - https://doi.org/10.21769/BioProtoc.2577
AB  - In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the ‘Hartig net’, where exchanges between the two partners take place. Finally, the efficiency of ectomycorrhizal fungi to improve the phosphorus nutrition of their host plants will depend on the regulation of phosphate transfer from the fungal cells to plant cells in the Hartig net through as yet unknown mechanisms. In order to investigate these mechanisms, we developed an in vitro experimental device mimicking the common apoplasm of the ectomycorrhizae (the Hartig net) to study the phosphorus metabolism in the ectomycorrhizal fungus Hebeloma cylindrosporum when the fungal cells are associated or not with the plant cells of the host plant Pinus pinaster. This device can be used to monitor 32Phosphate efflux from the fungus previously incubated with 32P-orthophosphate.
KW  - In vitro symbiotic interface
KW  - 32Phosphate efflux measurement
KW  - Hebeloma cylindrosporum
KW  - Pinus pinaster
KW  - Ectomycorrhizal symbiosis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False