TY  - JOUR
TI  - Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR
AU  - Kageyama, Daisuke 
VL  - 7
IS  - 19
PY  - 2017
DA  - 2017/10/05
SP  - e2566
C1  - Bio-protocol 2017;7:e2566
DO  - 10.21769/BioProtoc.2566
UR  - https://doi.org/10.21769/BioProtoc.2566
AB  - Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol for real-time qPCR based on SYBR Green I fluorescence as well as some tips to prevent possible pitfalls.
KW  - Bacteria
KW  - Endosymbionts
KW  - Insects
KW  - qPCR
KW  - Quantification
KW  - SYBR Green
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False