TY  - JOUR
TI  - Detection of Protein S-nitrosothiols (SNOs) in Plant Samples on Diaminofluorescein (DAF) Gels
AU  - Rodríguez-Ruiz, Marta  
AU  - Mioto, Paulo T. 
AU  - Palma, José  M. 
AU  - Corpas, Francisco J
VL  - 7
IS  - 18
PY  - 2017
DA  - 2017/09/20
SP  - e2559
C1  - Bio-protocol 2017;7:e2559
DO  - 10.21769/BioProtoc.2559
UR  - https://doi.org/10.21769/BioProtoc.2559
AB  - In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (King et al., 2005) for plant samples. Briefly, proteins from plant samples are separated via non-reducing SDS-PAGE, then the NO bound by S-nitrosylated proteins is released using UV light and, finally, the NO is detected using the fluorescent probe DAF-FM (Rodriguez-Ruiz et al., 2017). Thus, the approach presented here provides a relatively quick and economical procedure that can be used to compare protein SNOs content in plant samples and provide insight in NO-based signaling in plants.
KW  - Nitric oxide
KW  - S-nitrosothiols
KW  - S-nitrosation
KW  - S-nitrosylation
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False