TY  - JOUR
TI  - Polyadenylated RNA Sampling
AU  - Hajnsdorf, Eliane  
VL  - 2
IS  - 17
PY  - 2012
DA  - 2012/09/05
SP  - e254
C1  - Bio-protocol 2012;2:e254
DO  - 10.21769/BioProtoc.254
UR  - https://doi.org/10.21769/BioProtoc.254
AB  - Polyadenylation is a post-transcriptional modification of RNA occurring in prokaryotes, eukaryotes and organelles. However, the function and extent of bacterial polyadenylation are in marked contrast to those of eukaryotic poly(A) tails. In fact, the long poly(A) tails of eukaryotic mRNAs play an important role in their exportation to the cytoplasm and promote mRNA stability and translation, whereas the short bacterial tails facilitate RNA decay. One of the obstacles encountered by investigators studying bacterial polyadenylation is the scarcity of polyadenylated RNAs. The method described here allows reverse transcription and PCR amplification of the whole population of polyadenylated RNAs provided that the poly(A) tails are long enough to hybridize to oligo dT30 sequence. To this end utilization of exoribonucleases deficient strains may be useful.
KW  - Polyadenylation
KW  - Bacteria
KW  - Poly(A) tails
KW  - RNA degradation
KW  - Gene expression
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False