TY  - JOUR
TI  - Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from  Tgfbr2-deficient Squamous Cell Carcinoma
AU  - McCauley, Heather A
AU  - Guasch, Geraldine 
VL  - 7
IS  - 17
PY  - 2017
DA  - 2017/09/05
SP  - e2524
C1  - Bio-protocol 2017;7:e2524
DO  - 10.21769/BioProtoc.2524
UR  - https://doi.org/10.21769/BioProtoc.2524
AB  - Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation strategies must preserve their viability. The ability to isolate different populations of cells from the bulk of the tumor will continue to deepen our understanding of the biology of cancer stem cells. Here, we report the strategy combining mechanical tumor dissociation, enzymatic treatment and flow cytometry to isolate a pure population of epithelial cancer stem cells from their native microenvironment. This technique can be useful to further functionally profile the cancer stem cells (RNA sequencing and epigenetic analysis), grow them in culture or use them directly in transplantation assays.
KW  - Cancer stem cells
KW  - Flow cytometry
KW  - Cell isolation
KW  - Squamous cell carcinoma
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False