TY  - JOUR
TI  - Ribosome Fractionation in Yeast
AU  - Panasenko, Olesya O.
VL  - 2
IS  - 16
PY  - 2012
DA  - 2012/08/20
SP  - e251
C1  - Bio-protocol 2012;2:e251
DO  - 10.21769/BioProtoc.251
UR  - https://doi.org/10.21769/BioProtoc.251
AB  - This protocol describes yeast ribosome fractionation in the gradient of sucrose. During the cyclic process of translation, a small (40S) and large (60S) ribosomal subunit associate with mRNA to form an 80S complex (monosome). This ribosome moves along the mRNA during translational elongation, and then dissociates into the 40S and 60S subunits on termination. During elongation by one ribosome, further ribosomes can initiate translation on the same mRNA to form polysomes. The mass of each polysomal complex is determined primarily by the number of ribosomes it contains. Hence, the population of polysomes within the cell can be size-fractionated by sucrose density gradient centrifugation on the basis of the loading of ribosomes on the mRNA. Several compounds help to maintain or to disrupt the polysomes (Figure 1).
KW  - Ribosome
KW  - Polysomes
KW  - Translation
KW  - Gradient centrifugation
KW  - Yeast
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False