TY  - JOUR
TI  - Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
AU  - Cirera-Salinas, Daniel 
AU  - Ciaudo, Constance  
VL  - 7
IS  - 16
PY  - 2017
DA  - 2017/08/20
SP  - e2507
C1  - Bio-protocol 2017;7:e2507
DO  - 10.21769/BioProtoc.2507
UR  - https://doi.org/10.21769/BioProtoc.2507
AB  - A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation defects, we adapted this ‘exit from pluripotency assay’ (Bodak et al., 2017; Cirera-Salinas et al., 2017). The protocol described here has been successfully applied to several mESC lines and is easily transposable from one laboratory to another.
KW  - Mouse embryonic stem cells
KW  - Pluripotency
KW  - Commitment
KW  - 2i media
KW  - RNA interference
KW  - Alkaline phosphatase
KW  - in vitro assay
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False