TY  - JOUR
TI  - Assessment of Modulation of Protein Stability Using Pulse-chase Method
AU  - Elgendy, Mohamed  
VL  - 7
IS  - 16
PY  - 2017
DA  - 2017/08/20
SP  - e2443
C1  - Bio-protocol 2017;7:e2443
DO  - 10.21769/BioProtoc.2443
UR  - https://doi.org/10.21769/BioProtoc.2443
AB  - Pulse-chase technique is a method widely used to assess protein or mRNA stability. The principle of pulse-chase relies on labeling proteins or mRNA produced during a short period of time called ‘pulse’ and then following the rate of disappearance of those labeled proteins over a period of time called ‘chase’. This technique thus allows quantitative analysis of modulation of protein or mRNA stability under different treatments or culturing conditions.
KW  - Pulse-chase
KW  - Protein stability
KW  - Half-life
KW  - Protein degradation
KW  - Proteasome
KW  - Lysosome
KW  - MCL-1
KW  - mTOR
KW  - Sunitinib
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False