TY  - JOUR
TI  - RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells
AU  - Zhang, Yong-Wei  
AU  - Nasto, Rochelle E.
AU  - Jablonski, Sandra A.
AU  - Serebriiskii, Ilya G
AU  - Surana, Rishi 
AU  - Murray, Joseph 
AU  - Johnson, Michael 
AU  - Riggins, Rebecca B.
AU  - Clarke, Robert 
AU  - Golemis, Erica A.
AU  - Weiner, Louis M.
VL  - 7
IS  - 15
PY  - 2017
DA  - 2017/08/05
SP  - e2435
C1  - Bio-protocol 2017;7:e2435
DO  - 10.21769/BioProtoc.2435
UR  - https://doi.org/10.21769/BioProtoc.2435
AB  - RNAi screening technology has revealed unknown determinants of various biological signaling pathways in biomedical studies. This protocol provided detailed information about how to use RNAi screening to identify proliferation determinants in breast tumor cells. siRNA-based libraries targeting against Estrogen receptor (ER)-network, including 631 genes
relevant to estrogen signaling, was constructed for screening in breast cancer cells. Briefly, reverse transfection of siRNA induced transient gene knockdown in MCF7 cells. First, the transfection reagent for MCF7 cells was selected. Next, the Z’-score assay was used to monitor if screening conditions yielded efficiently. Then, the ER-network siRNA library screening was preceded by automatic machines under optimized experimental conditions.
KW  - RNA interference (RNAi)
KW  - Screening
KW  - Estrogen receptor (ER)
KW  - Breast cancer
KW  - Z’-score
KW  - Multidrop Combi-nL reagent dispenser
KW  - WellMate microplate dispenser
KW  - CyBio automatic dispenser
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False