TY  - JOUR
TI  - Stable Interference by shRNA with pSUPER.retro Vectors and Lipofectamine
AU  - Soddu, Silvia 
VL  - 2
IS  - 15
PY  - 2012
DA  - 2012/08/05
SP  - e243
C1  - Bio-protocol 2012;2:e243
DO  - 10.21769/BioProtoc.243
UR  - https://doi.org/10.21769/BioProtoc.243
AB  - RNA interference is a powerful genetic approach for efficiently silencing target genes. Expression of short hairpin RNAs (shRNAs) allows analysis of the consequences of stably silencing genes. This protocol describes a method to stably integrate shRNA constructs with pSUPER.retro vectors and Lipofectamine in RKO and H1299 cells. This method can be applied to cells from other lines with modification of drug selection and cell conditions. pSUPER.retro vectors can be also transfected into packaging cells by this same method to produce retroviral supernatants.
KW  - Interference
KW  - ShRNA
KW  - Transfection
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False