TY  - JOUR
TI  - Determination of Survival of Wildtype and Mutant Escherichia coli in Soil
AU  - Somorin, Yinka 
AU  - O'Byrne, Conor P.
VL  - 7
IS  - 14
PY  - 2017
DA  - 2017/07/20
SP  - e2414
C1  - Bio-protocol 2017;7:e2414
DO  - 10.21769/BioProtoc.2414
UR  - https://doi.org/10.21769/BioProtoc.2414
AB  - E. coli resides in the gastrointestinal tract of humans and other warm-blooded animals but recent studies have shown that E. coli can persist and grow in various external environments including soil. The general stress response regulator, RpoS, helps E. coli overcome various stresses, however its role in soil survival was unknown. This soil survival assay protocol was developed and used to determine the role of the general stress response regulator, RpoS, in the survival of E. coli in soil. Using this soil survival assay, we demonstrated that RpoS was important for the survival of E. coli in soil. This protocol describes the development of the soil survival assay especially the recovery of E. coli inoculated into soil and can be adapted to allow further investigations into the survival of other bacteria in soil.
KW  - Soil survival
KW  - Escherichia coli
KW  - RpoS
KW  - Environmental persistence
KW  - Bacteria recovery
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False