TY  - JOUR
TI  - The Sulfur Oxygenase Reductase Activity Assay: Catalyzing a Reaction with  Elemental Sulfur as Substrate at High Temperatures
AU  - Rühl, Patrick  
AU  - Kletzin, Arnulf 
VL  - 7
IS  - 14
PY  - 2017
DA  - 2017/07/20
SP  - e2403
C1  - Bio-protocol 2017;7:e2403
DO  - 10.21769/BioProtoc.2403
UR  - https://doi.org/10.21769/BioProtoc.2403
AB  - The sulfur oxygenase reductase (SOR) reaction is a dioxygen-dependent disproportionation of elemental sulfur (S0), catalyzed at optimal temperatures between 65 °C and 85 °C. Thiosulfate and sulfite are formed as oxidized products as well hydrogen sulfide as reduced product. External co-factors are not required. Usually, the SOR assay is performed in a milliliter scale in S0-containing Tris-buffer at high temperatures followed by colorimetric product quantification. In order to make the SOR assay more sensitive and better reproducible, several modifications were implemented compared to the original SOR assay (Kletzin, 1989). Here we present the modified SOR assay and the following quantification of the reaction products.
KW  - Sulfur oxygenase reductase
KW  - Elemental sulfur
KW  - Thermozyme
KW  - Sulfur disproportionation
KW  - Thiosulfate
KW  - Sulfite
KW  - Hydrogen sulfide
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False