TY  - JOUR
TI  - Microvesicle Isolation from Rat Brain Extract Treated Human Mesenchymal Stem Cells
AU  - Lee, Ji Yong  
AU  - Choi, Seong-Mi  
AU  - Kim, Han-Soo 
VL  - 7
IS  - 13
PY  - 2017
DA  - 2017/07/05
SP  - e2375
C1  - Bio-protocol 2017;7:e2375
DO  - 10.21769/BioProtoc.2375
UR  - https://doi.org/10.21769/BioProtoc.2375
AB  - Microvesicle (MVs) are submicron-sized membranous vesicles that are either actively released from cells via secretory compartments or shed from cell surface membranes. MVs are generated by many cell types and serve as vehicles that transfer biological information (e.g., protein, mRNA, and miRNA) to distant cells, thereby affecting their gene expression, proliferation, differentiation, and function. Although their physiological functions are not clearly defined, recent studies have shown their therapeutic potential for tissue repair and regeneration. While MVs can be isolated readily from mesenchymal stem cells (MSCs) and other cell types from various sources, the yield of MVs under conventional culture condition in vitro is one of the limiting factors for both the in vivo functional study as well as in vitro molecular analysis. Here, we provide a protocol to increase the yield of microvesicles by preconditioning MSCs with rat brain extract.
KW  - Mesenchymal stem cell
KW  - Microvesicle
KW  - Extracellular vesicles
KW  - Sucrose gradient
KW  - Diafiltration
KW  - Tissue regeneration
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False