TY  - JOUR
TI  - Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency
AU  - Rodriguez Ayala, Facundo 
AU  - Cogliati, Sebastián  
AU  - Bauman, Carlos  
AU  - Leñini, Cecilia  
AU  - Bartolini, Marco  
AU  - Villalba, Juan  Manuel 
AU  - Argañaraz, Federico  
AU  - Grau, Roberto  
VL  - 7
IS  - 12
PY  - 2017
DA  - 2017/06/20
SP  - e2345
C1  - Bio-protocol 2017;7:e2345
DO  - 10.21769/BioProtoc.2345
UR  - https://doi.org/10.21769/BioProtoc.2345
AB  - Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut worm, dilution is necessary before plating onto growth media. Serial dilutions can reduce the concentration of the original intestinal sample to levels low enough for single colonies to be grown on media plates, allowing for the calculation of the initial counts of bacteria in the intestinal sample.
KW  - Caenorhabditis elegans
KW  - Bacillus subtilis
KW  - Escherichia coli
KW  - Culture
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False