TY  - JOUR
TI  - RNA Capping by Transcription Initiation with Non-canonical Initiating Nucleotides (NCINs): Determination of Relative Efficiencies of Transcription Initiation with NCINs and NTPs
AU  - Bird, Jeremy  G. 
AU  - Nickels, Bryce  E. 
AU  - Ebright, Richard H.
VL  - 7
IS  - 12
PY  - 2017
DA  - 2017/06/20
SP  - e2336
C1  - Bio-protocol 2017;7:e2336
DO  - 10.21769/BioProtoc.2336
UR  - https://doi.org/10.21769/BioProtoc.2336
AB  - It recently has been established that adenine-containing cofactors, including nicotinamide adenine dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), and 3’-desphospho-coenzyme A (dpCoA), can serve as ‘non-canonical initiating nucleotides’ (NCINs) for transcription initiation by bacterial and eukaryotic cellular RNA polymerases (RNAPs) and that the efficiency of the reaction is determined by promoter sequence (Bird et al., 2016). Here we describe a protocol to quantify the relative efficiencies of transcription initiation using an NCIN vs. transcription initiation using a nucleoside triphosphate (NTP) for a given promoter sequence.
KW  - RNA polymerase
KW  - Transcription
KW  - Non-canonical initiating nucleotide (NCIN)
KW  - RNA capping
KW  - ab initio RNA capping
KW  - NAD+
KW  - NADH
KW  - 3’-desphospho coenzyme A
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False