TY  - JOUR
TI  - Functional Analysis of Connexin Channels in Cultured Cells by  Neurobiotin Injection and Visualization
AU  - Wörsdörfer, Philipp 
AU  - Willecke, Klaus 
VL  - 7
IS  - 11
PY  - 2017
DA  - 2017/06/05
SP  - e2325
C1  - Bio-protocol 2017;7:e2325
DO  - 10.21769/BioProtoc.2325
UR  - https://doi.org/10.21769/BioProtoc.2325
AB  - Functional gap junction channels between neighboring cells can be assessed by microinjection of low molecular weight tracer substances into cultured cells. The extent of direct intercellular communication can be precisely quantified by this method. This protocol describes the iontophoretic injection and visualisation of Neurobiotin into cultured cells.
KW  - Gap junction
KW  - Connexin
KW  - Neurobiotin
KW  - Microinjection
KW  - Iontophoresis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False