TY  - JOUR
TI  - Fluorescently Labelled Aerolysin (FLAER) Labelling of Candida albicans Cells
AU  - Singh, Sneh Lata
AU  - Komath, Sneha Sudha
VL  - 7
IS  - 11
PY  - 2017
DA  - 2017/06/05
SP  - e2303
C1  - Bio-protocol 2017;7:e2303
DO  - 10.21769/BioProtoc.2303
UR  - https://doi.org/10.21769/BioProtoc.2303
AB  - In this protocol we describe a nonradiolabelled labelling of GPI anchor in Candida albicans. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and can be carried out in vivo.
KW  - FLAER
KW  - GPI anchor
KW  - Candida albicans
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False