TY  - JOUR
TI  - Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum
AU  - Peyraud, Rémi 
AU  - Denny, Timothy P. 
AU  - Genin, Stéphane 
VL  - 7
IS  - 10
PY  - 2017
DA  - 2017/05/20
SP  - e2289
C1  - Bio-protocol 2017;7:e2289
DO  - 10.21769/BioProtoc.2289
UR  - https://doi.org/10.21769/BioProtoc.2289
AB  - Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of R. solanacearum.
KW  - Ralstonia solanacearum
KW  - Exopolysaccharide
KW  - Virulence factor
KW  - Plant pathogen
KW  - Hexoseamine
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False