TY  - JOUR
TI  - Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern Blotting
AU  - Bardelli, Martino  
AU  - Zarate-Perez, Francisco  
AU  - Agundez, Leticia  
AU  - Jolinon, Nelly 
AU  - Linden, R. Michael 
AU  - Escalante, Carlos R.
AU  - Henckaerts, Els 
VL  - 7
IS  - 9
PY  - 2017
DA  - 2017/05/05
SP  - e2271
C1  - Bio-protocol 2017;7:e2271
DO  - 10.21769/BioProtoc.2271
UR  - https://doi.org/10.21769/BioProtoc.2271
AB  - Adeno-associated virus (AAV) is a small single-stranded DNA virus that requires the presence of a helper virus, such as adenovirus or herpes virus, to efficiently replicate its genome. AAV DNA is replicated by a rolling-hairpin mechanism (Ward, 2006), and during replication several DNA intermediates can be detected. This detailed protocol describes how to analyze the AAV DNA intermediates formed during AAV replication using a modified Hirt extract (Hirt, 1967) procedure and Southern blotting (Southern, 1975).
KW  - Adeno-associated virus
KW  - AAV
KW  - DNA replication
KW  - Replicative intermediates
KW  - Southern blot
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False